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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Virology. 2013 Feb 26;439(1):23–33. doi: 10.1016/j.virol.2013.01.019

Figure 2. Ypet insertion into the HCV genome is unstable through serial passage.

Figure 2

(a) Longitudinal virus production in Huh-7.5 cells following electroporation of Jc1(Δ34-378-YPet), Jc1 5AB YPet or Jc1 5AB 2xYPet in which two copies of YPet separated by a GS-linker are inserted between NS5A and NS5B. (b) Mean fluorescence intensity of YPet following electroporation of Jc1(Δ34-378-YPet), Jc1 5AB YPet or Jc1 5AB 2xYPet into Huh-7.5 cells. (c–e) Genomic stability of (c) Jc1(Δ34-378-YPet), (d) Jc1 5AB YPet and (e) Jc1 5AB 2xYPet over multiple passages. Electroporated cells were passaged and cells were analyzed for the expression of NS5A and YPet by flow cytometry at the indicated time points. Results shown are means ± SD of three independent experiments.