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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Virology. 2013 Feb 26;439(1):23–33. doi: 10.1016/j.virol.2013.01.019

Figure 5. Viral characteristics of HCV with Gaussia luciferase inserted between NS5A and NS5B are maintained during infection of primary human hepatocytes.

Figure 5

(a) Infection frequency of primary human fetal hepatocytes with Jc1 5AB nsGluc or J6/JFH1 Clone 2 5AB nsGluc as determined by nuclear translocation in cells expressing tagRFPnlsMAVS. (b) Gaussia luciferase expression in cell lysates following infection of hepatocytes with Jc1 5AB nsGluc or J6/JFH1 Clone 2 5AB nsGluc with or without the NS5B inhibitor 2’CMA. (c) Representative images of infected hepatocytes indicated by nuclear translocation of the tagRFPnlsMAVS reporter. Data shown are mean ± SD of four independent experiments.