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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Virology. 2013 Feb 26;439(1):23–33. doi: 10.1016/j.virol.2013.01.019

Figure 6. Sensitive detection of HCV RNA replication of a monocistronic HCV genotype 1b genome containing a Gaussia luciferase gene between NS5A and NS5B.

Figure 6

HCV replication following electroporation of in vitro transcribed RNA of Jc1 5AB nsGluc or Con1 5AB ns Gluc into Huh-7.5 cells in the presence or absence of the NS5B polymerase inhibitor 2’CMA as measured by luciferase activity in cell lysates (a) or NS5A staining and flow cytometry (b). Representative images of Huh7.5 clone 8 cells electroporated with Jc1 5AB nsGluc (c, e) or Con1 5AB ns Gluc (d, f) indicated by nuclear translocation of the tagRFPnlsMAVS reporter at 100 (c, d) or 400 (d, f) fold magnification. Data shown are mean ± SD of three independent experiments.