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. Author manuscript; available in PMC: 2013 Apr 13.
Published in final edited form as: Cell. 2012 Apr 13;149(2):358–370. doi: 10.1016/j.cell.2012.01.053

Figure 2.

Figure 2

(A) Expression analysis of VSP-1 genes in WT and VC0177::Tn C6706 strains. The expression of each gene was determined relative to total 16S rRNA. 8 replicates were sampled for each gene. Significance was determined by t-test; * p < 0.05; ** p < 0.01; **** p < 0.0001. (B) VC0177 (VspR) ChIP enrichment of the 5′ UTR regions of VC0176, VC0178, VC0179 and VC0180 was determined by qPCR relative to sample DNA input. Enrichment of the promoter region of non VSP-1 gene VC1141 is shown as a control. Significance was determined by t-test relative to control enrichment; * p < 0.05; ** p < 0.01 (C) In vivo competition experiments measuring the ability of mutant strains to colonize the infant mouse intestine compared to the parental strain. Significance was determined by t-test relative to colonization ratio of parental strains wild type C6706 vs. C6706 ΔlacZ; **** p < 0.0001. See also Figure S1.