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. Author manuscript; available in PMC: 2013 Apr 8.
Published in final edited form as: Science. 2008 Jun 13;320(5882):1492–1496. doi: 10.1126/science.1158042

Figure 4. XBP1 directly regulates lipogenic genes in the liver.

Figure 4

(A) Expression of lipogenic genes in the liver of mice fed a standard chow diet was measured by quantitative real time PCR with actin mRNA as control. Values represent relative amounts of each mRNA compared to WT. Statistical significance of differences between WT and Xbp1Δ were determined. *, p<0.005; **, p<0.0001. (B) Primary hepatocytes were cultured in media containing indicated concentrations of glucose and insulin for 24 h. Nuclear extracts were subjected to western blot analysis. (C) Primary hepatocytes were infected with adenoviruses expressing XBP-1s or GFP control at 2 or 10 pfu per cell. mRNA levels were measured 24 hours after infection by real-time PCR. (D) CHIP assays were performed with liver nuclei of mice untreated or injected with Tm 6 hrs prior to sacrifice. Values represent fold increases of real-time PCR signals compared to the signal for the untreated WT CHIP with control serum.