Table 6. Western blot detection of the phyA2 protein in corn, diet, digesta, blood, tissues, and eggs of laying hens fed diets containing control corn (CC) and phytase transgenic corn (PTC) for 16 weeks¹.

Item	Number of positive samples		Positive detection frequency in CC or PTC fed laying hens (%)2
	CC	PTC	CC	PTC
Corn	0	8	0	100
Diet	0	8	0	100
Crop contents	0	8	0	100
Gizzard contents	0	7	0	87.5
Duodenal contents	0	3	0	37.5
Jejunum contents	0	1	0	12.5
Ileum contents	0	0	0	0
Cecal contents	0	0	0	0
Rectal contents	0	0	0	0
Blood	0	0	0	0
Heart	0	0	0	0
Liver	0	0	0	0
Spleen	0	0	0	0
Kidney	0	0	0	0
Breast muscle	0	0	0	0
Eggs	0	0	0	0

¹Number of samples that tested positive for the phyA2 protein out of 8 samples analyzed. One sample was tested per laying hen (n = 8 laying hens per treatment).

²Percentage of samples positive for the phyA2 protein taken from PTC fed laying hens i.e. (number of positive samples/number of samples tested)×100.