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. 2013 Apr 8;8(4):e61125. doi: 10.1371/journal.pone.0061125

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Flow cytometric analysis of 231-Empty and 231-GATA3 cells grown in full serum, SR (serum replacement) or SR +TGFß for 24 hrs. 231-GATA3 cells show a reduction in %S-phase cells upon TGF-ß1 treatment compared to SR conditions whereas 231-Empty cells continue to proliferate with TGF-ß1 treatment. (B) Western blot analysis of GATA3 downregulation by siRNA and flow cytometric analysis of TGFb effect in control or GATA-3 siRNA transfected cells. Percentage of cells in S-phase is provided on each panel.