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. 2013 Apr 8;8(4):e60455. doi: 10.1371/journal.pone.0060455

Figure 4. Elevated ERBB2 and ERBB3 expression in Female MMTV-NeuNT tumors.

Figure 4

A. Serum obtained at sacrifice from control (n = 11) and MARKO (n = 10) mice was analyzed for estradiol levels (E2). B. Serum from A was analyzed for testosterone (T) levels. C. MMTV-NeuNT expression. At the time of sacrifice, tumors were harvested and non-tumor bearing mammary glands were dissociated from the fat pad and RNA was prepared as described in the methods section. NeuNT expression was analyzed by quantitative RT-PCR and normalized to 18S expression. Expression of NeuNT in tumors (Control n = 9 and MARKO n = 7) for MARKO and Control mice and normal mammary glands (Control n = 26, MARKO = 15) was normalized to expression in non-tumor bearing mammary glands of the Control group. D-G. Immunohistochemical detection of ERBB2 in tumors, Control (D) and MARKO (E) at 40X magnification, and in normal mammary ducts, Control (F) and MARKO (G) at 100X magnification. Gene expression analysis of Erbb3 (H) and Erbb4 (I) in the same samples as in C. *denotes P<0.05, **P<0.01 and ***P<0.005.