Figure 3. Lower number of peripheral FoxP3⁺ Treg cells in aged Tg4 FoxP3^gfp males.

A. Frequency of FoxP3 expressing CD4⁺ T cells in the spleen of Tg4 FoxP3^wt and Tg4 FoxP3^gfp males aged 16–24 weeks (left) and the frequency of Helios and PD-1 expression gated on CD4⁺FoxP3⁺ splenocytes (middle and right). FoxP3^wt n = 9, FoxP3^gfp n = 6. Data represented as individual scores, with mean±SEM. B. Total number of splenocytes, CD4⁺ T cells or CD4⁺FoxP3⁺ Treg cells in the spleen. FoxP3^wt n = 9, FoxP3^gfp n = 6. Data represented as mean + SEM. C. Mean fluorescence intensity of FoxP3 staining in Treg cells in the spleen. FoxP3^wt, n = 9, FoxP3^gfp n = 6. Data represented as mean+SEM. D. Frequency of Foxp3 expression by CD4⁺ cells in the mLN. FoxP3^wt n = 4, FoxP3^gfp n = 3. Data represented as individual scores, with mean±SEM. E. Percentage of CD44^hi CD4⁺ T cells in the spleen. FoxP3^wt n = 9, FoxP3^gfp n = 6. Data represented as individual scores, with mean±SEM. P values represent significance determined by 2-tailed, unpaired student's t test. n.s. = not significant.