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. 2013 Apr 8;8(4):e59516. doi: 10.1371/journal.pone.0059516

Figure 4. S11 split intein trans-splicing in E. coli cells.

Figure 4

A. Schematic illustration of the trans-splicing reaction. Recombinant N-protein consists of a maltose binding protein sequence (M) followed by the large N-intein (IN). Recombinant C-protein consists of the small C-intein (IC) followed by a thioredoxin (T). B. Detection of trans-splicing. For each S11 split intein (specified on top), the N-protein and the C-protein were co-expressed in E. coli for overnight at 25°C, total cellular proteins were resolved by SDS-PAGE, and protein bands were visualized by Coomassie Blue staining or by Western blotting using an anti-T antibody as indicated. Positions are marked for the spliced protein (MT), the N-protein (MIN), and the C-protein (ICT).