Table 1. Primers used to quantify mRNA.
mRNA | Primers |
VEGF | (F) 5′-TACTGCTGTACCTCCACCTCCACCATG-3′ |
(R) 5′-TCACTTCATGGGACTTCTGCTCT-3′ | |
Ki67 | (F) 5′-CGG ACT TTG GGT GCG ACT T-3′ |
(R) 5′-GTC GAC CCC GCT CCT TTT-3′ | |
ADRP | (F) 5′-CACAAATTGCGGTTGCCAAT-3′ |
(R) 5′-ACTGGCAACAATCTCGGACGT-3′ | |
L-FABP | (F) 5′-CCATGAACTTCTCCGGCAAGT-3′ |
(R) 5′-TCCTTCCCTTTCTGGATGAGGT-3′ | |
ALPI | (F) 5′-TGAGGGTGTGGCTTACCAG-3′ |
(R) 5′-GATGGACGTGTAGGCTTTGCT-3′ |
VEGF, vascular endothelial growth factor; ADRP, adipocyte differentiation-related protein; L-FABP, liver fatty acid binding protein; ALPI, intestinal alkaline phosphatase; F, forward primer; R, reverse primer.