Figure 8. MHV nsp1 and nsp1-27D reduce the expression of reporter genes controlled by different promoters.

BHK-21 cells were co-transfected with pRLuc-CMV (CMV promoter), pGL3-control (SV40 promoter) or pLuc-ISRE (ISRE promoter), and the indicated expression plasmids. 24 h post transfection, the luciferase activity was measured. The results represent the means of at least four independent experiments. Statistical analysis was performed using paired Student's t-test (* p<0.01).