Figure 8.

Jagged2-mediated inhibition of TCR-β chain expression represses αβ-lineage differentiation. (A) Flow cytometric analysis of intracellular CD3ε and TCR-β expression in CD34⁺CD4⁻CD1a⁻ thymic progenitors after 3 wk of coculture on OP9 stromal cells expressing the different Notch ligands. Data are representative of three independent experiments. (B) The mean frequency of icTCR-β⁺ cells generated from CD34⁺CD4⁻CD1a⁻ thymic progenitors on the various OP9 stromal cells. Data are derived from three independent experiments (error bars indicate SEM, * = P < 0.05). (C–E) Flow cytometric analysis of human CD34⁺CD4^–CD1a^– thymic progenitors transduced with control, TCR-β, or TCR-δ after coculture on OP9 stromal cells expressing the different human Notch ligands, as indicated above the dot plots. Numbers in top right quadrants indicate the percentage of TCR-αβ⁺CD3⁺ cells (C), CD4⁺CD8β⁺ DP cells (D), or TCR-γδ⁺CD3⁺ cells (E). (F–H) Bar diagrams show the absolute numbers of TCR-αβ⁺CD3⁺ cells (F), CD4⁺CD8β⁺ DP cells (G), or TCR-αβ⁺CD3⁺ cells (H) obtained after OP9 coculture with the different Notch ligands. Error bars represent means and SEM of three independent experiments (D4 = DLL4, J1 = JAG1, and J2 = JAG2).