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. 2013 Apr 8;210(4):821–838. doi: 10.1084/jem.20122005

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© 2013 D’Antonio et al.

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Figure 4. — Reduction of Gadd34 levels results in increased eIF2α phosphorylation. (A) Quantitative RT-PCR for Gadd34 mRNA from P28 sciatic nerves. Error bars, SEM; *, P < 0.05; n = 4 RT from independent pools of nerves. (B) Western blot analysis on P28 sciatic nerve lysates was performed for Gadd34 and phosphorylated eIF2α; β-tubulin provides a control for loading. One representative experiment of four is shown. Numbers indicate relative molecular weights. (C) Gadd34 and (D) phosphorylated eIF2α protein levels as determined by densitometry. Error bars, SEM; **, P < 0.01, ***, P < 0.001; n = 4.