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. 2013 Apr 8;210(4):821–838. doi: 10.1084/jem.20122005

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© 2013 D’Antonio et al.

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Figure 7. — Increased translational attenuation in S63del/Gadd34^Δc/Δc nerves. (A) Western blot analysis on P28 sciatic nerves for phosphorylated eIF2α and ATF4. Numbers indicate relative molecular weights. One representative experiment of five is shown. (B) Phosphorylated eIF2α levels, as measured by densitometric analysis. Error bars, SEM; n = 5 repeat blots. (C) Measurement of newly synthesized proteins via S³⁵ incorporation (metabolic labeling) in explanted P28 WT, S63del, and S63del/Gadd34^Δc/Δc sciatic nerves. (E) Metabolic labeling measuring newly synthesized proteins in explanted sciatic nerves from P28 WT, Chop-null, and S63del/Chop-null nerves. 1 representative blot of 10 is shown for each experiment. (D and F). Quantification of newly synthesized proteins (via S³⁵ incorporation) by densitometric analysis. (F) Error bars, SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001, n.s., not significant, by Student’s t test; n = 10 independent experiments.