Table 2. Highly conserved miRNAs in Avicennia marina.
| MiRNAfamily | Sequence (5′-3′) | Length(nt) | Conserved in other plants | |||||||||||
| ath | ptr | bdi | gma | zm | osa | vvi | ||||||||
| miR156 | UGACAGAAGAGAGGGAGCAC | 20 | − | ++++ | − | − | − | − | ++++ | |||||
| UGACAGAAGAGAGUGAGCAC | 20 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||||||
| UUGACAGAAGAUAGAGAGCAC | 21 | − | ++++ | − | ++++ | − | − | ++++ | ||||||
| miR159 | UUUGGAUUGAAGGGAGCUCUA | 21 | ++++ | ++++ | − | ++++ | − | − | ++++ | |||||
| UUUGGAUUGAAGGGAGCUCUG | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| miR160 | UGCCUGGCUCCCUGUAUGCCA | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||||||
| UGCCUGGCUCCCUGUAUGCCG | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| miR164 | UGGAGAAGCAGGGCACGUGCA | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| miR166 | GGAAUGUUGGCUGGCUCGAGG | 21 | − | − | − | − | ++++ | ++++ | − | |||||
| GGAAUGUUGUCUGGCUCGAGG | 21 | − | − | − | ++++ | ++++ | ++++ | − | ||||||
| UCGGACCAGGCUUCAAUCCCU | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| UCGGACCAGGCUUCAUUCCC | 20 | − | − | − | ++++ | ++++ | − | − | ||||||
| UCGGACCAGGCUUCAUUCCCC | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||||||
| UCGGACCAGGCUUCAUUCCUC | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| UCUCGGACCAGGCUUCAUUCC | 21 | − | − | ++++ | ++++ | − | − | − | ||||||
| miR167 | UGAAGCUGCCAGCAUGAUCUA | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| UGAAGCUGCCAGCAUGAUCUG | 21 | − | ++++ | − | ++++ | ++++ | ++++ | ++++ | ||||||
| UGAAGCUGCCAGCAUGAUCUGA | 22 | − | − | ++++ | ++++ | − | − | − | ||||||
| UGAAGCUGCCAGCAUGAUCUU | 21 | − | ++++ | − | ++++ | − | − | − | ||||||
| miR168 | UCGCUUGGUGCAGAUCGGGAC | 21 | − | − | ++++ | − | ++++ | ++++ | − | |||||
| UCGCUUGGUGCAGGUCGGGAA | 21 | ++++ | ++++ | − | ++++ | − | − | ++++ | ||||||
| miR169 | CAGCCAAGGAUGACUUGCCGG | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| UAGCCAAGGAUGACUUGCCUA | 21 | − | ++++ | ++++ | − | ++++ | ++++ | ++++ | ||||||
| miR171 | UGAUUGAGCCGUGCCAAUAUC | 21 | − | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| UGUUGGCUCGGCUCACUCAGA | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| UUGAGCCGCGCCAAUAUCACU | 21 | − | − | − | ++++ | − | − | ++++ | ||||||
| UUGAGCCGUGCCAAUAUCACG | 21 | ++++ | ++++ | − | ++++ | − | − | − | ||||||
| miR172 | AGAAUCUUGAUGAUGCUGCA | 20 | − | − | − | ++++ | ++++ | − | − | |||||
| AGAAUCUUGAUGAUGCUGCAU | 21 | ++++ | ++++ | ++++ | ++++ | − | ++++ | − | ||||||
| miR319 | UUGGACUGAAGGGAGCUCCC | 20 | − | ++++ | − | ++++ | − | − | − | |||||
| UUGGACUGAAGGGAGCUCCCU | 21 | ++++ | − | − | ++++ | − | − | ++++ | ||||||
| UUGGACUGAAGGGUGCUCCC | 20 | − | − | − | − | ++++ | ++++ | − | ||||||
| miR390 | AAGCUCAGGAGGGAUAGCGCC | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| miR393 | UCCAAAGGGAUCGCAUUGAUC | 21 | − | ++++ | ++++ | ++++ | − | ++++ | ++++ | |||||
| UCCAAAGGGAUCGCAUUGAUCC | 22 | ++++ | − | − | ++++ | ++++ | − | − | ||||||
| UCCAAAGGGAUCGCAUUGAUCU | 22 | − | − | − | − | ++++ | ++++ | − | ||||||
| miR394 | UUGGCAUUCUGUCCACCUCC | 20 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | |||||
| miR396 | GGUCAAGAAAGCUGUGGGAAG | 21 | − | − | − | − | ++++ | ++++ | − | |||||
| UUCCACAGCUUUCUUGAACUG | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ||||||
| UUCCACAGCUUUCUUGAACUU | 21 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | − | ||||||
| miR397 | UCAUUGAGUGCAGCGUUGAUG | 21 | ++++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | |||||
| miR398 | UGUGUUCUCAGGUCACCCCUU | 21 | ++++ | ++++ | − | ++++ | − | ++++ | ++++ | |||||
| UGUGUUCUCAGGUCGCCCCUG | 21 | − | ++++ | ++++ | ++++ | − | ++++ | ++++ | ||||||
| miR399 | UGCCAAAGGAGAAUUGCCCUG | 21 | − | ++++ | ++++ | − | ++++ | ++++ | ++++ | |||||
| UGCCAAAGGAGAGUUGCCCUA | 21 | − | ++++ | − | − | − | ++++ | − | ||||||
| UGCCAAAGGAGAGUUGCCCUG | 21 | ++++ | − | − | ++++ | ++++ | ++++ | ++++ | ||||||
| UGCCAAAGGAGAUUUGCCCGG | 21 | ++++ | ++++ | − | − | − | − | ++++ | ||||||
| miR403 | UUAGAUUCACGCACAAACUCG | 21 | ++++ | ++++ | − | − | − | − | ++++ | |||||
| miR408 | AUGCACUGCCUCUUCCCUGGC | 21 | ++++ | ++++ | − | ++++ | − | − | ++++ | |||||
| CUGCACUGCCUCUUCCCUGGC | 21 | − | − | − | − | ++++ | ++++ | − | ||||||
| miR828 | UCUUGCUCAAAUGAGUAUUCCA | 22 | − | ++++ | − | ++++ | − | − | ++++ | |||||
| miR2111 | UAAUCUGCAUCCUGAGGUUUA | 21 | ++++ | − | − | ++++ | − | − | − | |||||
The abbreviations represent the following: ath, Arabidopsis thaliana; ptr, Populus trichocarpa; bdi, Brachypodium distachyon; gma, Glycine max; zma, Zea mays; osa, Oryza sativa; vvi, Vitis vinifera. ++++, miRNA sequences of mangrove that were exactly identical to those of other species; -, miRNA sequences of mangrove that were not present in other species.