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In vitro activation by anti-MPO IgG of neutrophils. Neutrophils from B6 (black circles), 129S6 (black triangles), and 129S1 (black squares) mice were primed with TNF-α, incubated with murine anti-MPO IgG, and superoxide generation was measured as an indicator of neutrophil activation. Negative control experiments included neutrophils from B6 (white circles), 129S6 (white triangles), and 129S1 (white squares), all treated with TNF-α, but no anti-MPO IgG.
