Table 1.

Sequences of the Primers Used for Quantitative Gene Expression Analysis

Gene	Target	Primer sequence
GJA1	Cx43m	5′-CAATCACTTGGCGTGACTTC-3′
		5′-AACGAAAGGCAGACTGCTCA-3′
	Cx43p	5′-TTGCAATCTGTGATCCTTGAA-3′
		5′-AACGAAAGGCAGACTGCTCA-3′
GJC1	Cx45m	5′-ATCTGGAAAAATTGCAATCAAAA-3′
		5′-CTGTAAGGACGATCCGGAAG-3′
	Cx45p	5′-TGGGTAACCGAAGTTCTGGA-3′
		5′-TCCAGGATCCAGGTAAAAACC-3′
GJA3	Cx46m	5′-GCCGGCCAGTACTTTCTGTA-3′
		5′-CCTGCTTGAGCTTCTTCCAG-3′
	Cx46p	5′-ATGCCTGTCCTGTGGAGAAG-3′
		5′-GAAGATGAACAGCACGGTCA-3′
GJB2	Cx26m	5′-CTTCCTCCCGACGCAGAG-3′
		5′-GCTCATCTCCCCACACCTC-3′
GJA5	Cx40m	5′-AAGTCCAGGGAGGAGGAAAA-3′
		5′-TCGTATCACACCGGAAATCA-3′
GJB1	Cx32m	5′-TGCAGACATTCTCTGGGAAA-3′
		5′-TCTCATCACCCCACACACTC-3′
HPRT-1		5′-TTGAGTTTGGAAACATCTGGAG-3′
		5′-GCCCAAAGGGAACTGATAGTC-3′

The PCR products size is approximately 200 bp.

m, mRNA; p, primary transcript.