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. 2013 Apr;182(4):1248–1254. doi: 10.1016/j.ajpath.2012.12.024

Figure 3.

Figure 3

GRP blockade diminishes pSmad2/3+ cells in mouse lung at 10 weeks after radiation. A: Sham plus PBS group. B: Radiation plus PBS group. C: Radiation plus 77427 group. D: Image analysis. A–C: Nuclear pSmad2/3 immunostaining (black arrows) was significantly elevated at 10 weeks after radiation (compared with negative nuclei counterstained with methyl green, white arrows). Sham controls had relatively few pSmad2/3+ cells; then the relative numbers of these cells increased more than fourfold after radiation. ∗P < 0.001 for radiation plus PBS versus sham plus PBS (fourfold increase) or radiation plus 77427; †P < 0.01 for radiation plus 77427 was only 30% elevated over sham plus 77427; ‡P < 0.001 for sham controls given only 77427 had a doubling of pSmad2/3+ cells versus the sham plus PBS group. Scale bars: 25 μm (A–C). V, blood vessel.