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. 2013 Apr;182(4):1434–1447. doi: 10.1016/j.ajpath.2012.12.003

Figure 4.

Figure 4

Effect of IL-1β expression on leukocytes in trachea of CCSP/IL-1β mice. A–C: Whole mounts of tracheas stained for macrophages/dendritic cells (Aif1/Iba1, red) and neutrophils (S100A8, green). A: At baseline (no Dox), few leukocytes stain for AIf1/Iba1, but some (arrows) have clear dendritic morphology. Scale bar = 50 μm. B: Large increase in leukocytes stained for S100A8 (green) and Aif1/Iba1 (red), mostly with rounded phenotype (arrowheads) at 2 weeks of Dox. C: Reduction in leukocyte number after 2 weeks of Dox, followed by 2 weeks of normal water. Cells staining for Aif1/Iba1 and with dendritic morphology re-appear (arrows). D–I: Staining for macrophages/dendritic cells (red) and VEGF-C (green) in single- or double-transgenic mice given Dox for 2 weeks. Diffuse granular staining with the polyclonal antibody to VEGF-C was found in the epithelium of all tracheas. D–F: Single-transgenic CCSP mice. D: Many cells staining for Aif1/Iba1 have a dendritic morphology E: Only a few (arrow) have weak staining for VEGF-C (arrow). F: Merged image. G–I: Double-transgenic CCSP/IL-1β mice. G: Most cells staining for Aif1/Iba1 have a rounded macrophage-like phenotype. H: Some of these macrophages have stronger VEGF-C staining (arrowheads). I: Merged image.