Table 2.
Effect of IL-1β Overexpression in Mouse Trachea on Expression of Selected Genes for Chemokines and Their Receptors, Leukocyte-Related Genes, Extracellular Matrix Proteases, Inflammation-Related Genes, and Lymphatic Markers
| Symbol | Name | Fold change∗ | Adjusted P value† |
|---|---|---|---|
| Chemokines and receptors | |||
| Cxcl1 | Chemokine (C-X-C motif) ligand 1 | 20.80 | 0.00009 |
| Ccl7 | Chemokine (C-C motif) ligand 7 | 7.06 | 0.00008 |
| Ccl17 | Chemokine (C-C motif) ligand 17 | 4.91 | 0.00036 |
| Ccl9 | Chemokine (C-C motif) ligand 9 | 4.76 | 0.00001 |
| Cxcr2/Il8r1 | Chemokine (C-X-C motif) receptor 2 | 3.57 | 0.00190 |
| Cxcl14 | Chemokine (C-X-C motif) ligand 14 | 3.07 | 0.00004 |
| Ccr6 | Chemokine (C-C motif) receptor 6 | 3.01 | 0.00001 |
| Cxcl16 | Chemokine (C-X-C motif) ligand 16 | 2.53 | 0.00001 |
| Leukocyte related | |||
| Lcn2 | Lipocalin 2 (neutrophil-gelatinase associated lipocalin) | 37.81 | 5.67×10−8 |
| S100a9 | S100 calcium binding protein A9 (calgranulin B) | 14.46 | 0.00002 |
| S100a8 | S100 calcium binding protein A8 (calgranulin A) | 6.65 | 0.00051 |
| Ncf4 | Neutrophil cytosolic factor 4 | 6.08 | 5.42×10−6 |
| Selp | P-selectin | 5.14 | 0.00037 |
| Selplg | P-selectin ligand | 4.62 | 4.75×10−6 |
| Aif1 | Allograft inflammatory factor 1 (Aif1/Iba1) | 4.36 | 0.00001 |
| Cd68 | CD68 antigen | 2.98 | 0.00009 |
| Extracellular matrix proteases (remodeling related) | |||
| Mmp3 | Matrix metallopeptidase 3 (stromelysin, progelatinase) | 8.90 | 0.00012 |
| Ctse | Cathepsin E | 3.86 | 0.00079 |
| Mmp12 | Matrix metallopeptidase 12 | 2.79 | 0.00460 |
| Inflammation and asthma related | |||
| Clca3 | Chloride channel calcium activated 3 | 61.91 | 4.85×10−5 |
| Chi3l4 | Chitinase 3-like 4 | 42.46 | 6.39×10−7 |
| Saa3 | Serum amyloid A3 | 34.57 | 1.73×10−6 |
| Chi3l1 | Chitinase 3-like 1 | 16.19 | 2.13×10−5 |
| Muc5ac | Mucin 5, subtypes A and C, tracheobronchial/gastric | 13.18 | 1.25×10−6 |
| Muc13 | Mucin 13 | 11.03 | 7.82×10−7 |
| Clca4 | Chloride channel calcium activated 4 | 10.74 | 3.65×10−6 |
| Interleukin 1 related | |||
| Il1b | Interleukin 1 beta (mouse gene) | 11.52 | 0.00002 |
| Il1rn | Interleukin 1 receptor antagonist | 4.86 | 0.00001 |
| Il1r2 | Interleukin 1 receptor, type II | 3.09 | 0.00005 |
| TNF family related | |||
| Ltb | Lymphotoxin-beta | 7.32 | 0.00002 |
| Tnf | Tumor necrosis factor alpha | 3.50 | 0.00005 |
| Lta | Lymphotoxin-alpha | 2.10 | 0.00377 |
Gene expression in tracheas of bi-transgenic CCSP/IL-1β mice given Dox for 2 weeks as determined by Illumina genome-wide gene array chips; n = 3 mice per group.
∗
Fold changes compared with control bi-transgenic mice given normal water.
†
P values adjusted to compensate for false discovery rate in large microarray samples.