Figure 7.

Representative photomicrographs showing the effect of UPR blockade and/or VEGF neutralization on vascular lesion formation in the CNV and OIR mouse models. A: Representative photomicrographs of the vascular lesion quantified in Figure 6, C and D. Mice underwent the CNV model, and animals were terminated at 14 days after laser injury. Lesions were labeled with vascular-specific dye, Ricinus Communis Agglutinin I, conjugated to rhodamine, and lesions were visualized by confocal microscopy. Mice received a 1-μL intravitreal injection of 2 μg per eye siRNAs against IRE1α, ATF6, or CRYAB, 5 μmol/L MG132, or 50 μmol/L AEBSF in the presence or absence of 5 ng VEGF164 neutralizing antibody at laser injury. Scrambled siRNA (scr) acted as a control. B: Representative photomicrographs of the vascular lesion quantified in Figure 6, E and F. Mouse pups underwent the OIR model, and animals were terminated at P17. Retinal sections were prepared and imaged. Mouse pups received a 0.5-μL intravitreal injection of 1 μg per eye siRNA against IRE1α, ATF6, CRYAB, 5 μmol/L MG132, or 50 μmol/L AEBSF in the presence or absence of 5 ng VEGF164 neutralizing antibody at P12 (immediately after being removed from the hypoxic chamber). Scale bar = 20 μm.