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. 2013 Apr 8;8(4):e61288. doi: 10.1371/journal.pone.0061288

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© 2013 Bose et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Flow cytometric analysis on permeabilized and non-permeabilized (i) SP2/O and (ii) B16F10 cells using pooled antisera from βhCG-TT (black profiles) and βhCG-TT + MIP (green profiles) immunized mice; pre-immune sera (blue profiles) served as control. Filled red profiles: Secondary antibody controls. (B) Right panels: Immunofluorescence analysis on permeabilized COLO205 cells depicting reactivity of (i) pre-immune sera, (ii) antisera derived from βhCG-TT + MIP immunized mice and (iii) antisera derived from βhCG-TT immunized mice. Left panels: Corresponding phase contrast images. Bars = 30 µm.