Figure 4.

Tumor response to single and combination treatments. A: Tumor growth curves. LPSX1 showed slowed growth when treated with rapamycin or combination treatment, but LPSX2 and LPSX3 did not show slowed tumor growth in response to any of the treatments. B: Histological analysis shows evidence of treatment response, including necrosis and morphological changes suggestive of differentiation. Representative images of LPSX3 (H&E stain) at low (top row) and high magnification (middle and bottom rows) show differentiation and necrotic areas. Boxed areas correspond (although not to scale) to the high-power images of differentiation (black boxes) and necrosis (red boxes). Data are expressed as means ± SEM. n = 3 or 4 per treatment group. Scale bars: 2.5 mm (low-power images); 50 μm (high-power images).