Figure 1.

Experimental design and isolation of UM and B/i cells from normal bladder urothelium. A: Representative immunohistochemical analyses of UPKII and CK5 in normal urothelium; prominent UM cells (arrows). Scale bar = 50 μm. B: Microphotograph of frozen tissue section was taken with PALM Microbeam IV Laser Capture Microscope (LCM; Zeiss). UM, B/i, and CIS samples were microdissected and analyzed with both miRNA and mRNA microarrays. MP samples were macrodissected (MD) and analyzed only in the miRNA microarray. C: Delineation of UM cells, before and after laser capture. Scale bar = 75 μm. D: RT-qPCR on UM and B/i cells for UPKII and CK5. Graph shows B/i log₂ fold expression; B/i expression normalized to UM. Horizontal line indicates the sample median. Square indicates the sample mean. *P < 0.05 by Student’s t-test.