Fig. 4.

AAD-2004 partially reversed the down-regulated expression of calbindin in the brain of Tg-βCTF99/B6 mice. (A-F) Photomicrographs showing anti-calbindin-stained hippocampus of non-transgenic control mice (WT; A, B), control Tg-βCTF99/B6 mice (TG; C, D), and Tg-βCTF99/B6 mice fed with AAD-2004 (TG+AAD-2004; E, F). Photomicrographs with high magnification of rectangles on the left panels (A, C, E) were shown (B, D, F). Note the anti-calbindin immunoreactivity not only in the granular layer (g) of the dentate gyrus, but also in the pyramidal cells (arrows), in the molecular layer (ml) of the dentate gyrus, and in the stratum oriens (so) and stratum radiatum (sr) of the hippocampus. p, pyramidal layer; hi, hilus. Scale bar, 200 µm. (G) Representative Western blot images showing the expression of mouse amyloid precursor protein (mAPP), COX-2, calbindin, transthyretin (TTR), phospho-CREB, and β-actin in the hippocampus. Total 5-8 animals were analyzed for each group.