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. 2013 Apr 8;8(4):e60770. doi: 10.1371/journal.pone.0060770

Figure 6. Effect of anziaic acid on type IB human topoisomerase I activity.

Figure 6

(a) Assay of relaxation activity –1 U of enzyme was used in each relaxation reaction with 250 ng of supercoiled plasmid DNA. (b) DNA cleavage assay with 5 U of enzyme. Gel electrophoresis was carried out in the presence of 0.5 µg/mL ethidium bromide and camptothecin was used as a positive control at 62.5 µM (C1) or 125 µM (C2). -: no enzyme. S: supercoiled plasmid DN substrate. N: nicked plasmid DNA. Ro: Relaxed closed plasmid DNA. PR: partially relaxed plasmid DNA. CC: Covalently closed circular DNA.