Skip to main content
. 2013 Apr 8;8(4):e60770. doi: 10.1371/journal.pone.0060770

Figure 7. Effect of anziaic acid on E. coli DNA gyrase activity.

Figure 7

(a) Assay of supercoiling activity with 1 U of enzyme and 250 ng of relaxed plasmid DNA substrate. (b) DNA cleavage assay with 5 U of enzyme. Gel electrophoresis was carried out in the presence of 0.5 µg/mL ethidium bromide. Positive controls – F: 100 µM levofloxacin; N: 100 µM norfloxacin. S: supercoiled plasmid DNA substrate. N: nicked plasmid DNA. Ro: Relaxed closed plasmid DNA. PR: partially relaxed plasmid DNA. L: linear DNA CC: covalently closed circular DNA.