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(A) IL-6 levels were determined by ELISA in the plasma of mice bearing i.p. OVCAR-3 tumors, 4 and 24 h post minocycline treatment (single dose 30 mg/kg; i.p.) (B) Tumors harvested at 4 and 24 h were examined by western blot analysis for the expression of IL-6, p-STAT3 (Tyr 705), MCL-1 and p-ERK1/2 protein. (*p<0.05, **p<0.01 and ***p<0.001 vs. control).
