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. 2013 Apr;2(2):118–127. doi: 10.1089/biores.2012.0300

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 4. — The monocyte chemoattractant protein-1 (MCP-1) promoter was amplified from bisulfite-converted DNA and analyzed by nested methylation-specific polymerase chain reaction (nMS-PCR). MCP-1 promoter DNA hypomethylation in mice (A), statistical analysis of MCP-1 promoter DNA hypomethylation (B), serum MCP-1 levels in mice (C), correlation of MCP-1 hypomethylation and atherosclerosis area (D) (r=0.5175, p=0.0006, n=10). M, amplified band by methylation-specific primer; U, amplified band by unmethylation-specific primer. Values were means±SEM. *p<0.05 vs. blank control, n=10.