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. 2013 Apr 8;8(4):e60028. doi: 10.1371/journal.pone.0060028

Figure 6. Willin antagonizes some of the genes regulated by YAP in sciatic nerve fibroblasts.

Figure 6

mRNA expression of the YAP target genes CTGF, BMP2, FGF1, RASSF8, IGFBP3 and PRL was probed in fibroblasts expressing Willin or YAP by quantitative real-time PCR. mRNA levels were compared with the empty vector control (set to 1). Willin overexpression increased BMP2, FGF1, RASSF8, IGFBP3 and PRL mRNA levels and, together with YAP, decreased CTGF mRNA expression in these cells. Means were calculated from Ct values in three independent experiments. β-actin was used to normalize for variances in input cDNA. Error bars represent ±s.e. (n = 3). Fibroblasts-vector vs fibroblasts-Willin or fibroblasts-YAP for all the analysed genes: *p<0.05; **p<0.01; ***p<0.001; Student’s t-test. Fibroblasts-vector vs fibroblasts-YAP: BMP2 (p = 0.217); Student’s t-test.