FIGURE 3.

Effects of pharmacological inhibitors on BZLF1 expression in Raji cells. Raji cells were treated with vehicle (Cont), 10 μM DZNep, 300 nMTSA, 1 μM 5-Aza alone or in combinations as indicated. As a positive control (TPA/A/Bu), Raji cells were treated with TPA/A23187/butyrate at 20 ng/ml, 1 μM and 5 mM, respectively. For DZNep or 5-aza-2′-deoxycytidine (5-Aza) treatment, cells were exposed to the reagent daily for 3 days. Treatment with other chemicals was for 24 h. Real-time RT-PCR was carried out to measure the levels of BZLF1 mRNA, which were then normalized to GAPDH mRNA levels. The light-blue arrows indicate that DZNep alone did not efficiently induce BZLF1, but could markedly enhance the expression if treated in combination with TSA.