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. 2013 Apr 8;8(4):e60981. doi: 10.1371/journal.pone.0060981

Figure 4. Relative levels of CCP mRNAs in selected tissues of WT and pcd mice.

Figure 4

mRNA levels of CCP1 through 6 in heart and spleen (panels A and B, respectively) of adult WT and mutant mice were analyzed using quantitative real-time PCR. CCP1 mRNA is the most abundant transcript in the heart and spleen of WT animals. The levels of CCP1 transcript are decreased in pcd mice, but the levels of other CCP transcripts are not affected. Fold change in expression was calculated using the DDCt method. GAPDH was used as an internal control. n = 3 for each genotype. *, p<0.05 using Student’s t-test. NS, not significant.