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Contact cytotoxicity of bovine pericardium with porcine aortic smooth muscle cells. We confirmed cross-linking efficiency in vitro. Phase-contrast microscopy image of contact cultured cells with pericardium (original magnification ×40). Cells were labeled with Annexin V fluorescein isothiocyanate (FL1) and propidium iodide (FL2), then identified using flow cytometry.
