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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Jan 30;190(5):2282–2291. doi: 10.4049/jimmunol.1201215

Fig. 2. Type I NKT cells are activated by A.a.-infected DCs.

Fig. 2

Murine BMDCs were infected with A.a. (panel A) or P.g. at indicated MOI for 24 hrs in the presence (shaded bars) of blocking CD1d antibodies (clone 1B1, 20 μg/mL) or isotype controls (black bars). Liver MNC were added as responder cells and co-cultured with DCs for further 48 hrs. IL-4 and IFNγ levels in supernatants were determined 24 and 48h later, respectively, by ELISA. C. Murine BMDCs were infected with A.a. or P.g. or cultured in the presence or absence of LPS for 24 hrs. Histograms show CD86 expression on MHC-II+CD11c+ cells. Data shown are representative of three experiments. n=3; *, p<0.05; **, p <0.01; ***, p<0.001.