Table 2.

Average RINs and RNA yield (μg) from biobank samples.

Cohort	n	Percent RIN > 6	RIN (average ± SD)	RNA yield (mean ± SD)
EPIC-Italy	64	95	7.1 ± 0.7	3.9 ± 1.7
NSHDS	50	92	7.4 ± 0.9	12.2 ± 7.5
The EPIC-Italy sample set included six samples stored at –80oC with a RIN of 6.8 ± 0.5 and RNA yield of 5.1 ± 1.2. The remaining samples were stored in liquid nitrogen. The NSHDS sample set included nine samples with EDTA as anticoagulant with a RIN of 6.7 ± 0.8 and RNA yield of 13.9 ± 6.8 μg. The remaining samples used heparin. EPIC-Italy and NSHDS yields were obtained from half a cryostraw or half a microcentrifuge tube of buffy coat, corresponding to ~ 0.25 and 0.7–1.0 mL buffy coat (corresponding to ~ 3 and ~ 9 mL blood), respectively.