Table 2.

Oligonucleotides used for RT-PCR, qRT-PCR, mutant verification, site directed mutagenesis (target mutated nucleotides are underlined) and in vitro transcription (for the forward primers, the T7 promoter sequence is underlined and the bases incorporated into RNA during transcription are in bold). f indicates forward primer and r indicates reverse primer.

Purpose/Name	Sequence (5′ to 3′)
RT-PCR and qRT-PCR
ghoS-f	CCATCATCTTATTCCTCAGTGTTCT
ghoS-r	TAAGTCTAAGCATTTGAGCCTGATT
ghoT-f	TGGTGTGAACATATCCTTTGTCA
ghoT-r	TAATGCCACAGGCAGACTCA
ralR-f	AAACCATGTCCATTTTGTGGT
ralR-r	TCCAGTGGTTCGTTTATTCCA
ompA-f	CACTGGCTGGTTTCGCTACCG
ompA-r	ACCCATTTCAAAGCCAACATC
ompF-f	AAGCGCAATATTCTGGCAGT
ompF-r	TGCCACCGTAACTGTTTTCA
yciG-f	ACATCGTGGTGGTTCAGGAA
yciG-r	TACCACCGCTTTGTTGACCG
Deletion mutant verification
GhoS-veri-f	CTCCTATATGAGAATCATCAATCGGGG
GhoS-veri-r	GACAAAGGATATGTTCACACCAATCAC
GhoT-veri-f	GACCTCAACATTATGACAGTTGATGAC
GhoT-veri-r	GCTTCGTTCATCGTTCCGCAAATCCAG
Site-directed mutagenesis
GhoT-GCU-1-f	GTGATTGGTGTGAACATAAGCTTTGTCATTATCTGGTTTATCTC
GhoT-GCU-1-r	GAGATAAACCAGATAATGACAAAGCTTATGTTCACACCAATCAC
GhoT-GCU-2-f	CACATATTCGTTTACTTAGTGCTTTCCTGGTCGGAATAACCTGG
GhoT-GCU-2-r	CCAGGTTATTCCGACCAGGAAAGCACTAAGTAAACGAATATGTG
T7 in vitro transcription
PT7-GhoT-f	TAATACGACTCACTATAGGGAGAATGGCACTATTCTCTAAAATATTAATTTTT
PT7-GhoT-r	CTAAAAGAGAGAAAAAAGTAATGC
PT7-GhoS-f	TAATACGACTCACTATAGGGAGAATGGAAGGTAAAAACAAGTTCAATACTTAT
PT7-GhoS-r	TCATCAACTGTCATAATGTTGAG