Table 2. Effect of benzamidine and benzamidine-derivatives (applied at 20 μM concentration) on embryo mortality induced by inoculation of fertilized eggs with P. gingivalis(4 × 106cfu/egg).
Results are shown as: numbers of dead eggs in the presence of inhibitor/number of dead eggs in the absence of inhibitor(% of reduction of dead eggs by addition of an inhibitor)
| Number of dead eggs with/without inhibitor | Total number of eggs with/without inhibitor |
||||||
|---|---|---|---|---|---|---|---|
|
| |||||||
| 0 h | 1 h | 2 h | 3 h | 5 h | 24 h | ||
| 2,6-bis-(4-amidinobenzyl)-cyclohexanone* | |||||||
| Controls | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 24/24 |
| ATCC 33277 | 0/0 (0%) | 2/4 (50%) | 2/7 (71%) | 10/16 (38%) | 10/18 (45%) | 11/18 (39%) | 24/24 |
| M5-1-2 | 0/0 (0%) | 2/7 (71%) | 6/11 (45%) | 16/17 (6%) | 17/19 (11%) | 21/22 (5%) | 24/24 |
| Pentamidine | |||||||
| Controls | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 24/24 |
| ATCC 33277 | 0/0 (0%) | 1/3 (67%) | 5/8 (38%) | 13/15 (13%) | 14/18 (22%) | 16/19 (16%) | 24/24 |
| M5-1-2 | 0/0 (0%) | 5/6 (17%) | 7/10 (30%) | 12/18 (33%) | 13/21 (38%) | 16/21 (24%) | 24/24 |
| Benzamidine | |||||||
| Controls | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 0/0 (0%) | 24/24 |
| ATCC 33277 | 0/0 (0%) | 2/3 (33%) | 5/7 (24%) | 15/15 (0%) | 19/19 (0%) | 18/18 (0%) | 24/24 |
| M5-1-2 | 0/0 (0%) | 5/7 (29%) | 10/10 (0%) | 17/18 (6%) | 20/20 (0%) | 19/19 (0%) | 24/24 |
*
None of the inhibitors by themselveswere toxic to eggs