Table 1.
Selected list of TNFAIP8 interacting proteins identified by antibody microarray analysis
| ID | Symbol | Fold Change1 | P-value2 | Entrez Gene Name | Location | Type(s) |
|---|---|---|---|---|---|---|
| P56159 | GFRA1 | 2 | 4.00E-05 | GDNF family receptor alpha 1 | Plasma Membrane | transmembrane receptor |
| P09874 | PARP1 | 1.8 | 4.00E-05 | poly (ADP-ribose) polymerase 1 | Nucleus | enzyme |
| Q01130 | SRSF2 | 1.5 | 4.00E-06 | serine/arginine-rich splicing factor 2 | Nucleus | transcription regulator |
| P52292 | KPNA2 | 1.5 | 1.90E-04 | karyopherin alpha 2 (RAG cohort 1, importin alpha 1) | Nucleus | transporter |
| Q9UHI6 | DDX20 | 1.4 | 1.90E-04 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 | Nucleus | transcription regulator |
| P18031 | PTPN1 | 1.3 | 1.50E-04 | protein tyrosine phosphatase, non-receptor type 1 | Cytoplasm | phosphatase |
| P24864 | CCNE1 | 1.2 | 4.00E-05 | cyclin E1 | Nucleus | transcription regulator |
1
PC-3 cells were treated with TNFAIP8 siRNA (Si2 SiRNA/SiRNA) or a scrambled siRNA (ScrSiRNA/MM) and the proteomic profiles in the TNFAIP8 immune-complexes were identified by the antibody microarray analysis as described in Materials and Methods. Detailed list of all proteins is provided in Supporting Information Table 1. Fold change represents the ratio of largest of MM. Si, divided by smallest.
2
P-value is the significance of the difference between the two spots for MM and the two spots for SiRNA. All results shown above were found to be statistically significant even under the very stringent Bonferroni criterion to adjust for multiple testing.