Skip to main content
. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Sci Transl Med. 2013 Mar 13;5(176):176ra32. doi: 10.1126/scitranslmed.3005191

Fig. 5.

Fig. 5

ICOS+CXCR3+CXCR5+CD4+ T cells are specific for influenza antigens. (A) Ki67 expression by ICOS+CXCR3+CXCR5+CD4+ T cells at day 7 after vaccination (n = 3). A representative result is shown on the top panels. (B) CD154 assay. PBMCs obtained at day 7 after vaccination were stimulated with either Fluzone or heat-killed PR8 influenza virus for 6 hours. CD154+ cells represent antigen-specific cells. Gated to CXCR5+CD4+ and CXCR5−CD4+ T cells. A representative from experiments with four donors. (C) The percentage of CD154+ cells within ICOS+ and ICOS− cells among the CXCR5+ and CXCR5−CD4+ T cells upon stimulation with Fluzone (top) and PR8 virus (bottom). n = 4. ***P < 0.001, one-way ANOVA.