Figure 2.

T cell proliferation in response to AA antigens. DLN cells from each of the three groups, nontreated (mean spontaneous proliferation [MSP] = 159 cpm), pcDNA3-vaccinated (MSP = 198 cpm), and CD25-vaccinated (MSP = 223 cpm) were pooled from three rats, and their responses were measured on day 22 after AA induction. Stimulating antigens were PPD or the p180 peptide of Mt HSP65. Proliferative responses are presented as the SI ± SEM of quadruplicate cultures. Proliferation to PPD of DLN cells from CD25-vaccinated rats was significantly higher (P = 0.003) than that of pcDNA3-vaccinated rats. This is a representative experiment of three repetitions.