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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Anal Chem. 2013 Feb 5;85(4):2208–2215. doi: 10.1021/ac3029344

Figure 4.

Figure 4

ECμPs as platforms for protein capture assays in flow cytometry. Note: (▲)denotes ECμPs with capture antibody and (■) denotes particles without capture antibody. (a) ECμPs used in a sandwich assay for prostate specific antigen (PSA) in physiological buffer (PBS). (b) ECμPs used in a binding assay for goat anti-mouse IgG-phycoerythrin performed in 10% volume porcine plasma diluted with PBS. Note: all data points were obtained from triplicate experiments analyzed by gated flow cytometric analysis. Error bars represent the standard deviation of the mean for 3 separate determinations of median fluorescence intensity.