Skip to main content
. 2004 Mar 15;113(6):895–904. doi: 10.1172/JCI19852

Figure 1.

Figure 1

Regulation of MAT1A by SMases. (A) Cultured rat hepatocytes were treated with TNF-α, with or without D-threo-PDMP (PDMP), or with hASMase or bNSMase alone for 16 hours. MAT1A and MAT2A mRNA levels were analyzed by Northern blot and were normalized to 18S mRNA content, and the corresponding ratio was expressed as percentage of control. The plots in the graph (bottom) are the mean ± SD of six individual experiments. *P < 0.05 versus control; denotes significance for MAT1A and MAT2A changes. (B) These experiments were similar to those shown in A except that MAT1A mRNA levels were determined by real-time PCR analyses as described in Methods. Results are expressed as the mean ± SD of three individual experiments. *P = 0.05 versus control. (C) MAT I/III protein content was determined by Western blot after 18 hours of incubation with TNF-α, hASMase, or bNSMase at the dose shown. Data are the mean ± SD of three individual experiments. *P = 0.05 versus control. C, control.