Sequence and structural comparison of TIFY family members. (A) Structural organization of the identified P. vulgaris TIFY genes. Conserved motifs: CCT, TIFY, divergent CCT, Jas and GATA zinc-finger, are depicted in colored boxes assigned as indicated at the bottom of the panel. The genes are denoted by their species (Pv) abbreviation followed by the gene name; transcript IDs [4] are also provided. (B) WebLogo [38] of the putative TIFY and JAS motifs constructed from appropriate subsets of the sequence alignment of 23 bean TIFY sequences with Arabidopsis homologs. For the Jas motif analysis the TIFY2 family, which lacks this motif, was excluded. (C) Phylogenetic tree of the TIFY family. Protein sequences were aligned using ClustalX. A neighbor-joining tree was constructed using the PHYLIP package from 18, 58 and 19 amino acid sequences of TIFY family members from A. thaliana, G. max and P. vulgaris, respectively. The branches are color-coded to indicate their phyletic association to the Arabidopsis TIFY classification. A green, red or blue dot at the end of each branch indicates genes from A. thaliana, G. max or P. vulgaris, respectively.