Figure 5.

Transcript profiles of PvTIFY-RNAi and PvTIFY-OE transgenic roots compared with EV (control) transgenic roots. MapMan maps of processes belonging to the regulation overview (A), stress response (B) and metabolism overview (C) categories are shown. In each map, the left panel shows PvTIFY-RNAi/EV expression ratios and the right panel shows PvTIFY-OE/EV, as indicated. Up- or down-regulated ESTs are false color-coded with increasing blue or red, respectively, saturating at amplitude of 0.1 (log₂ value) as indicated in the bar from panel B. ESTs with no significant change in amplitude are shown in white. Expression ratios of selected ESTs obtained from microarrays (maps) were validated by qRT-PCR analysis. Normalized data from qRT-PCR gene expression assays are shown in bar-graphs at the bottom of panels A, B and C. The ESTs selected for qRT-PCR analysis correspond to the circled squares from each map and are indicated with black and white arrowheads. The arrowheads in the maps correspond to those in the bar-graphs. The ESTs ID [46] are indicated in the x-axes of the bar-graphs. In each pair of bars, the blue bar corresponds to the PvTIFY-RNAi/EV expression ratio (left in the maps) and the red bar corresponds to the PvTIFY-OE/EV expression ratio (right in the maps). The annotations for the selected ESTs are mentioned in the text.