Table 1.
Selected P-responsive genes identified by microarray analysis
| |
|
|
Normalized fold change (log 2) -P/C |
||
|---|---|---|---|---|---|
| Gene | BinName | ID a | EV | PvTIFY-RNAi | PvTIFY-OE |
|
Induced in control roots and repressed in PvTIFY-RNAi roots | |||||
| Cell wall invertase |
Major CHO |
TC8333 |
0.132 |
−0.076 |
0.196 |
| Seed imbibition, hydrolase |
Minor CHO |
EC997013, CV535625, EC911359 |
0.158 |
−0.053 |
0.160 |
| Phosphoenolpyruvate carboxylase |
Gluconeogenesis/ glyoxylate cycle |
TC12305, FE897651, FE711062 |
0.140 |
−0.120 |
0.069 |
| Malic enzyme |
TCA / org. Transformation |
TC19528 |
0.120 |
−0.038 |
0.122 |
| Alanine-glyoxylate transaminase |
Amino acid metabolism |
EC911309, CV537351, TC15378 |
0.112 |
−0.095 |
0.189 |
| Inorganic diphosphatase/ pyrophosphatase |
Nucleotide metabolism |
TC9801 |
0.102 |
−0.021 |
0.099 |
| Signal transducer/ triacylglycerol lipase |
Stress |
TC13450 |
0.108 |
−0.067 |
0.113 |
| Cold regulated 413 plasma membrane 1 |
Stress |
TC17916 |
0.164 |
−0.012 |
0.151 |
| Trypsin and protease inhibitor family protein |
Stress |
TC10263 |
0.142 |
−0.010 |
0.413 |
| Cytochrome P450 /CYP705A2) |
Miscellaneous enzyme families |
TC17341 |
0.149 |
−0.024 |
0.125 |
| Glutathione S-transferase |
Miscellaneous enzyme families |
TC14044 |
0.199 |
−0.023 |
0.375 |
| inositol-polyphosphate 5-phosphatase |
DNA |
TC17029, TC18885 |
0.145 |
−0.002 |
0.064 |
| CCAAT-binding transcription factor (CBF-B/NF-YA) family protein |
RNA |
TC11100 |
0.102 |
−0.036 |
0.057 |
| AP2 domain-containing TF |
RNA |
TC17374 |
0.103 |
−0.046 |
0.123 |
| Homeobox protein 6 |
RNA |
TC18879 |
0.120 |
−0.023 |
0.058 |
| Protein phosphatase 2C, putative |
Protein |
TC8583 |
0.110 |
−0.045 |
0.045 |
| Protein kinase 2A |
Protein |
TC8739 |
0.113 |
−0.016 |
0.086 |
| ATPase, coupled to transmembrane movement of substances |
Transport |
TC15570, TC9579 |
0.120 |
−0.028 |
0.153 |
| Putative phosphate transporter 1 (PHO1) |
Transport |
CV543807 |
0.105 |
−0.015 |
0.022 |
| Hypersensitive-induced response protein |
Transport |
TC10794 |
0.145 |
−0.031 |
0.181 |
|
Repressed in control roots and induced in PvTIFY-RNAi roots |
|
||||
| Fasciclin-like arabinogalactan protein, putative |
Cell wall |
TC8476 |
−0.095 |
0.180 |
−0.042 |
| Xyloglucan endotransglucosylase/hydrolase 9 |
Cell wall |
TC12404 |
−0.102 |
0.047 |
−0.057 |
| Acetoacetyl-coa thiolase |
Lipid metabolism |
TC9630 |
−0.101 |
0.064 |
−0.152 |
| Lipid-transfer protein, non-specific |
Lipid metabolism |
FE898308 |
−0.109 |
0.005 |
−0.025 |
| delta-8 sphingolipid desaturase |
Lipid metabolism |
TC15181 |
−0.050 |
0.127 |
−0.118 |
| Ovule/fiber cell elongation protein |
Hormone metabolism |
TC18895 |
−0.105 |
0.108 |
−0.142 |
| GAST1 Protein homolog 4 |
Hormone metabolism |
TC18314 |
−0.143 |
0.146 |
−0.097 |
| Histone H4 |
DNA |
TC14696 |
−0.101 |
0.088 |
−0.234 |
| Histone H3 |
DNA |
TC13898 |
−0.106 |
0.099 |
−0.186 |
| ATHB13; DNA binding / transcription factor |
RNA |
TC15258 |
−0.122 |
0.047 |
−0.125 |
| MYB, transcription factor |
RNA |
BQ481439 |
−0.187 |
0.017 |
−0.022 |
| DNA binding / transcription factor |
RNA |
TC15305 |
−0.102 |
0.002 |
−0.069 |
| Y14 RNA binding protein |
RNA |
CV538348 |
−0.101 |
0.022 |
−0.007 |
| Remorin family protein |
RNA |
TC18087 |
−0.103 |
0.007 |
−0.012 |
| Eukaryotic translation initiation factor 4 F, putative |
Protein |
TC13072 |
−0.100 |
0.004 |
−0.102 |
| 60S ribosomal protein L14 (RPL14B) | |
Protein |
TC14174, TC17981, TC12005, TC10014, TC12381 |
−0.095 |
0.130 |
−0.397 |
| Receptor-like protein kinase-related |
Signaling |
CV532262 |
−0.116 |
0.004 |
−0.077 |
| RKL1 (Receptor-like kinase 1) |
Signaling |
TC11321 |
−0.107 |
0.105 |
−0.234 |
| RHO-Like GTP binding protein 4 |
Signaling |
TC18636 |
−0.124 |
0.039 |
−0.057 |
| Calreticulin 2 | Signaling | TC16682 | −0.004 | 0.107 | −0.167 |
a. From bean gene index [46], ESTs encoded by the same gene present in the microarray and with similar differential expression are shown.