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. 2004 Mar 15;113(6):846–855. doi: 10.1172/JCI19900

Figure 3.

Figure 3

Histological analysis of the proximal tibiae of PPARγ+/– and WT littermates. (A) Histological features at proximal tibiae of PPARγ+/– and WT littermates. Villanueva-Goldner staining, calcein double labeling, and TRAP staining were done at 8 weeks; toluidine blue staining was done at 52 weeks of age. In Villanueva-Goldner staining, mineralized bone is stained green and unmineralized bone osteoid red; scale bar: 100 μm. In calcein double labeling, the mineralization front is stained as a green line; scale bar: 10 μm. In TRAP staining, TRAP-positive osteoclasts are stained red; scale bar: 100 μm. In toluidine blue staining, adipocytes are observed as oval vacuoles; scale bar: 50 μm. (B) Histomorphometric parameters at 8 weeks of age. Ob.S/BS, percentage of bone surface covered by cuboidal osteoblasts; OS/BS, percentage of bone surface covered by osteoid; MAR, mineral apposition rate; BFR, bone formation rate expressed by MAR times percentage of bone surface exhibiting double labels plus one-half single labels; Oc.N/B.Pm, number of mature osteoclasts in 100 mm of bone perimeter; ES/BS, percentage of eroded surface. Data are expressed as means (bars) ± SEMs (error bars) for eight mice per group for PPARγ+/– and WT mice. *Significant difference from WT, P < 0.01.