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. 2013 Jan 10;10:19. doi: 10.1186/1743-422X-10-19

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Copyright © 2013 Chung et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Titration of progeny viruses. Titer of progeny viruses produced from the cell was measured by TCID50 assay in 384-well plate format with 4 wells per dilution of virus. Ten (10) μL of 10-fold serial dilutions of progeny virus containing medium from respective samples (drug treated or untreated) were used to infect fresh Hep-2 cells in a 384-well format. After 6 days incubation the inhibitory effects of the compound and drug were assessed using Cell Titer Glo. A well showing a luminescence signal less than the mean of the non-infected control signal minus five times the standard deviation of the control was regarded as positive for infection.