Table 4.
Comparison between the differential expressions measured using DNA microarrays and qRT-PCR
| |
|
|
Pressure |
Pressure oxygen |
||||
|---|---|---|---|---|---|---|---|---|
| DNA microarrays | qRT-PCR | DNA microarrays | qRT-PCR | |||||
| ID |
Name |
Gene |
FC |
Adj. P-val |
FC |
FC |
Adj. P-val |
FC |
| PP_4265 |
transcriptional regulator Anr |
anr |
+ 1.6 |
1.2 E-02 |
+ 1.3 |
- 2.0 |
2.9 E-02 |
- 2.6 |
| PP_2257 |
aerotaxis receptor Aer-1 |
aer-1 |
+ 2.8 |
4.8 E-05 |
- 3.5 |
+ 2.6 |
1.1 E-05 |
- 1.7 |
| PP_2258 |
sensory box protein |
|
- 1.5 |
1.6 E-02 |
- 1.6 |
- 1.6 |
3.2 E-02 |
- 2.2 |
| PP_0504 |
outer membrane OprG |
oprG |
+ 2.5 |
5.8 E-04 |
+ 3.9 |
- 3.4 |
3.1 E-04 |
- 9.4 |
| PP_3087 |
excinuclease ABC A subunit putative (type VI) |
|
+ 1.6 |
3.1 E-03 |
+ 1.4 |
+ 2.3 |
1.7 E-03 |
+ 1.9 |
| PP_0104 |
cytochrome c oxidase subunit I |
|
n. s. |
- 2.3 |
- 1.7 |
5.6 E-03 |
- 4.8 |
|
| PP_4255 |
cytochrome c oxidase cbb3-type, subunit I |
ccoN-2 |
- 1.4 |
3.0 E-02 |
- 1.7 |
- 1.5 |
7.4 E-04 |
- 1.9 |
| PP_4870 |
azurin |
azu |
+ 2.2 |
1.9 E-03 |
+ 2.0 |
- 1.2 |
2.0 E-01 |
- 1.9 |
| PP_2439 | alkyl hydroxide reductase C subunit | ahpC | + 1.9 | 3.8 E-03 | + 2.1 | + 3.9 | 2.5 E-05 | + 3.7 |
Nine of the most relevant genes affected by elevated pressure (Pressure) and elevated pressure and dissolved oxygen (Pressure Oxygen) were tested. Differential expression is given in fold change (FC) where positive and negative values indicate how many times the gene transcription is increased and decreased, respectively, compared to the Control. The significance of differential expression is given by the adjusted P-value (Adj. P-value).
n. s. = no significant differential expression measured.