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High performance liquid chromatography analysis of biotransformation of p-coumaric acid in BL21 (DE3) harboring Os4CL and NtHST. A, p-coumaric acid (S1); B, reaction products of p-coumaric acid (P1 - P4); C, mass spectrometry (MS) data for the four reaction products. The mass spectrometer was operated in negative mode. The molecular masses of p-coumaric acid and shikimic acid are 164 Da and 174 Da, respectively.
